Dr. Arnd Pralle

Photo of Associate Professor Arnd Pralle  

Associate Professor, Ph.D. LMU Munich (1999)

Office: 229 Fronczak Hall,  (716) 645-3069
Lab: 146 & 150 Fronczak Hall (716) 645-2677
Email: apralle@buffalo.edu
link to personal website for more info

Education

  Ph.D. -- Ludwig-Maximilians-University, Munich (1999)
M.S. (Dipl.) -- Justus-Liebig-University, Giessen (1994)

Research Interests : Cellular and Molecular Biophysics

 
  • We investigate the
    • Mechanical dynamics and structure-function of membrane proteins
    • Lipid membrane domains and nanoscale diffusion or membrane proteins
    • Quantification and modeling of spatio-temporal anistropies inside cells
  • by applying and development methods like
    • Single molecule fluorescence,
    • Thermal-noise imaging and optical trapping
    • Fluorescence lifetime imaging

 

Enzymes as macromolecular machines undergo conformational changes in the background of thermal fluctuations. Few methods are available to study the mechanics of this dynamics, esp. in the case of membrane proteins. We are developing single molecule methods to study the mechanics and structure-function relationships of membrane proteins using fluorescence and laser trap based thermal-noise imaging.

Cellular processes such as organogenesis, tissue function, and synaptic plasticity require spatio-temporal patterning at the subcellular level. We develop assays based on single molecule fluorescence, fluorescence lifetime imaging and thermal-noise imaging to address the dynamics of spatio-temporal patters in membranes and inside cells.

Selected Publications

  1. "Analysis of a RanGTP-regulated Gradient in Mitotic Somatic Cells.", P.Kalab*, A.Pralle*, E.Y. Isacoff, R. Heald, and K. Weis, Nature. 440, 697 (2006).
  2. "Determination and Correction of Position Detection Nonlinearity in Single Particle Tracking and Three-dimensional Scanning Probe Microscopy.", C. Tischer, A. Pralle, and E.L. Florin, Microsc Microanal. 10, 425 (2004).
  3. "A Selective, Cell-permeable Optical Probe for Hydrogen Peroxide in Living Cells.", M.C. Chang, A. Pralle, E.Y. Isacoff, and C.J. Chang, J Am Chem Soc. 126, 15392 (2004).
  4. "The Orientation and Molecular Movement of a K(+) Channel Voltage-Sensing Domain.", C.S.Gandhi, E.Clark, E. Loots, A. Pralle, and E.Y. Isacoff, Neuron. 40, 515 (2003).
  5. "Sphingolipid-cholesterol Rafts Diffuse as Small Entities in the Plasma Membrane of Mammalian Cells.", A. Pralle, P. Keller, E.L. Florin, K. Simons, and J.K. Horber, J Cell Biol. 148, 997 (2000).